Rhodamine dUTP and capillary eletrophoresis

chovek69 via methods%40net.bio.net (by ivanoov from gmail.com)
Wed Dec 12 05:56:59 EST 2007


Yeah Wolfgang makes a good point. Capillary electrophoresis is very
sensitive to salt concentrations above the regular PCR ones (e.g in
restrictase buffers). Try desalting or diluting samples 1/5 or 1/10
with ddH2O before separation.

Hope this will help

On Dec 12, 10:07 am, WS <novalidaddr... from nurfuerspam.de> wrote:
> Dear Ed,
>
> have you tried desalting or doing a (PCR) cleanup before you analyze
> the samples?
>
> Best regards,
>
> Wolfgang



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