Rhodamine dUTP and capillary eletrophoresis

[chovek69]

Yeah Wolfgang makes a good point. Capillary electrophoresis is very
sensitive to salt concentrations above the regular PCR ones (e.g in
restrictase buffers). Try desalting or diluting samples 1/5 or 1/10
with ddH2O before separation.

Hope this will help

On Dec 12, 10:07 am, WS <novalidaddr... from nurfuerspam.de> wrote:
> Dear Ed,
>
> have you tried desalting or doing a (PCR) cleanup before you analyze
> the samples?
>
> Best regards,
>
> Wolfgang