Rhodamine dUTP and capillary eletrophoresis

[Pragnya Das]

Hi everyone
I'm facing a serious cloning issue.

I'm trying to clone a gene into an RNAi vector (pRFP ~6kb), by PCR and then
subclone it into RCAS (~11kb) vector. Even after cutting out the insert from
RFP, followed by gel purification, i'm still getting the insert cloned into
RFP, although I ligate my insert in RCAS. Why is this happening? has anyone
faced a problem like this before?

Pragnya Das