Rhodamine dUTP and capillary eletrophoresis

Pragnya Das via methods%40net.bio.net (by drpragnyadas from gmail.com)
Fri Dec 14 14:06:14 EST 2007


Hi everyone
I'm facing a serious cloning issue.

I'm trying to clone a gene into an RNAi vector (pRFP ~6kb), by PCR and then
subclone it into RCAS (~11kb) vector. Even after cutting out the insert from
RFP, followed by gel purification, i'm still getting the insert cloned into
RFP, although I ligate my insert in RCAS. Why is this happening? has anyone
faced a problem like this before?

Pragnya Das


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