removing bound SDS from protein samples

Larisa via methods%40net.bio.net (by larisa_troitskaya from yahoo.com)
Mon Dec 17 19:39:28 EST 2007


Hi experts,

I need very pure protein for FACE analysis. As last stage I'm going to use
purification from SDS-PAGE: cut out single band from the gel and then
extract protein. I'm afraid that SDS (still bound to my protein of interest
after  extraction) will interfere with PNGase F digestion (though reaction
mixture contains SDS at final concentration 0.05%). Could you, please,
suggest method to remove SDS from my protein sample after extraction
SDS-protein complex from gel ??? By now I have found only info on GeBa kit,
but it needs lots of buffer substitutions, and I could end up with
unsufficient amount of protein.

Thank you,
Larisa
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