western blot

Han via methods%40net.bio.net (by nobody from nospam.not)
Tue Dec 18 21:10:06 EST 2007

WS <novalidaddress from nurfuerspam.de> wrote in news:1479089b-0cc1-4051-aff6-
a2dd19d1b1c7 from d4g2000prg.googlegroups.com:

> Valeria,
> if you haven't loaded your samples yet on the gel, just add 5 or 10ul
> 2M DTT, it will work fine. If you already have run the gel, don't
> panic (too much), you just did not reduce your samples. They will run
> at different sizes maybe. You also might see some addional bands due
> to cross-linking and inter-/intramolecular -SS- bridges.
> Best,
> Wo

In addition, some antibodies don't recognize reduced proteins - they need a 
"native", folded protein configuration.

Best regards
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