IHC Related Problem

[StewJW]

Could it just be your primary antibody causing the problem? Zymed
(Invitrogen) sells a primary mouse anti-GFAP antibody, catalogue
08-0021,  which comes in a range of concentrations to work with your
own development system or you can use one of Zymeds kits, which have a
low background and very high sensitivity, The Histostain Plus Kit is a
peroxidase DAB kit for mouse primary antibodies, catalogue 85-9143.
These kits use a biotin labeled secondary antibody followed by a
peroxidase labelled strepatavidin. The reagents come in dropper
bottles and no reconstitution or pipetting is required, and they are
designed specificially for IHC.

Link for primary antibody product note:
http://www.invitrogen.com/content/sfs/manuals/08-0021_Rev%201107.pdf