Methods Digest, Vol 31, Issue 17- Re: transport s bacterial strain

Virash Gupta via methods%40net.bio.net (by virashkgupta from gmail.com)
Mon Dec 31 03:09:19 EST 2007


This is common requirement and practice amongst scientists who move amongst
different countries. I am using following methods.
1. Dispense LB-agar in 1.5 ml sterile microcentrifuge tubes, let them
solidify in verticle position to make stabs. You can add antibiotic if you
know your culture has resistance for it in media before dispensing (this
will keep contaminants in check). Pick small amount of overnight grown
culture with a sterile loop and insert the needle vertically into solidified
stab. Close the tube and carry as amany as you can in small space. Ypu can
easily retrieve the culture for upto 15 days if carried at RT or upto 3
month if stored at 4 degree C, after picking small growth from the top of
stab from inside thestab by streaking on the plate. It will not fail. LB
allows growth of most organisms- or you can use optimal medium.
2. Alternately mix one volume of good liquid culture with half volume of 50%
sterile growth. close the tube and carry.
Shuold not be difficult/
all the best
Dr V K Gupta
Professor of Microbiology and Mol Biology
PAU, Ludhiana
virashkgupta from gmail.com

On Dec 29, 2007 10:34 PM, <methods-request from oat.bio.indiana.edu> wrote:

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>   1. Re: removing bound SDS from protein samples (Larisa)
>      (Tay liang xin)
>   2. transport s bacterial strain (sarita mallik)
>   3. Re: transport s bacterial strain (DK)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 28 Dec 2007 11:55:35 +0800
> From: "Tay liang xin" <liangxin28 from gmail.com>
> Subject: Re: removing bound SDS from protein samples (Larisa)
> To: methods from oat.bio.indiana.edu
> Message-ID:
>        <42b3ff70712271955h47116dbfja7d294d098c0691b from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear Larisa,
>
> Maybe you can refer to this journal doi: 10.1016/j.jmb.2007.11.026 to
> solve
> your problem.
> "Refolding SDS-Denatured Proteins by the Addition of Amphipathic
> Cosolvents"
>
> Regards
> Liangxin
> INFORMM, USM
> Malaysia
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 28 Dec 2007 11:16:26 +0530
> From: "sarita mallik" <sarita81 from gmail.com>
> Subject: transport s bacterial strain
> To: methods from magpie.bio.indiana.edu
> Message-ID:
>        <cbd6f8dc0712272146m5d16bc4cjbb8446d1ae6b2d1 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear All,
> I 'm a research scholar in India. i have to go to Germany for a 6 month
> reserch fellowship. for this i need to carry my bacterual strain (non
> pathogenic) along with me. can anybody suggest the guidelines for export
> of
> biological material .
>
> thanks
>
> Sarita
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 28 Dec 2007 20:50:45 GMT
> From: dk from no.email.thankstospam.net (DK)
> Subject: Re: transport s bacterial strain
> To: methods from net.bio.net
> Message-ID: <Jkddj.60$Eq2.33 from newsfe02.lga>
>
> "sarita mallik" <sarita81 from gmail.com> wrote:
> >Dear All,
> >I 'm a research scholar in India. i have to go to Germany for a 6 month
> >reserch fellowship. for this i need to carry my bacterual strain (non
> >pathogenic) along with me. can anybody suggest the guidelines for export
> of
> >biological material .
>
> Regulations in India, Germany and airlines aside, this can be
> as trivial as taking a diluted LB culture into screw cap eppendord
> and putting it in the pocket. Or you can bring a streaked plate with
> you. Or a lyophilized sample (add just about any sugar to >1M
> and lyophilize).
>
> But, because of the regulations crap, I'd probably send the culture
> by Fedex to a colleague in Germany.
>
> DK
>
>
>
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> End of Methods Digest, Vol 31, Issue 17
> ***************************************
>



-- 
Dr V K Gupta
Sr Microbiologist (Molecular Biology)
Insect Molecular Biology Lab
Department of Entomology
Punjab Agricultural University
Ludhiana (Pb)-141004- India
M: 09815963210


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