hk2 cells culture

WS via methods%40net.bio.net (by novalidaddress At nurfuerspam.de)
Thu Feb 1 05:42:49 EST 2007


I observed this with othe cells (porcine renal mesangial cells,
primary cultures) . It seems to be related to the surface structure of
the dish. You might try to coat the dish with

a) gelatin. just pipet an aqueous solution of gelatin (eg 1g/liter) in
the dish and remove it 10 min later. Then plate your cells.
b) collagen. a cheap method is to extract rat tails with 1M HOAc over
a several days, cover the surface of the dish with this solution and
evaporate the solvent in vacuo. Quite tedious procedure, so you might
try gelatin first.

HTH Wo



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