DNA concentrations for megaprimer annealing and extension
(by customer At newsnet.com)
Thu Feb 1 06:35:40 EST 2007
> Dear experts,
> I would like to anneal two partially complementary pieces of DNA
> obtained by pcr (about 500 bp each with a 50bp overlap) and extend
> them (adding dNTP and Pol). So each strand will compete for the
> complementary strand an the other strand with the overlapping region.
> Is there a concentration range where this might work best?
> Next step would be a PCR amplification with end-to-end primers.
> Your input is welcome!
> Best regards,
200ng for each primer. For your next step, use 5-10ng DNA.
More information about the Methods