RT-PCR problem

TieQiao Wu via methods%40net.bio.net (by TieQiao.Wu At baker.edu.au)
Sun Feb 4 18:34:53 EST 2007


According to my experience, (1) dilute your template, 2ul work well for Taq, you can try 2ul or 1ul for Pfu because the RNase inhibitor can affect the activity of DNA polymerase (2) try different Mg2+ concentration
Good luck

Tieqiao Wu




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