Blunt end ligation

Ricardo via methods%40net.bio.net (by nightfallelf from yahoo.com)
Wed Feb 14 12:28:29 EST 2007


Thank you for your answers guys. I'm doing it today.

> 2. Circularization of the insert is not a problem,
since insert does
> not have origin of replication.

Its not a question of if it replicates or not
originating false positives in the plates, the problem
is if it recircularizes it wont be able to ligate to
the vector, right? that's why i was concerned about
it.

Thanks

Ricardo


 
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