Blunt end ligation

Ricardo via (by nightfallelf from
Wed Feb 14 12:28:29 EST 2007

Thank you for your answers guys. I'm doing it today.

> 2. Circularization of the insert is not a problem,
since insert does
> not have origin of replication.

Its not a question of if it replicates or not
originating false positives in the plates, the problem
is if it recircularizes it wont be able to ligate to
the vector, right? that's why i was concerned about



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