Simple question that is hard to answer

peter via methods%40net.bio.net (by peter.ianakiev from gmail.com)
Thu Feb 15 21:38:41 EST 2007


On Feb 15, 4:51 pm, "Carlo Zambonelli" <zambo... from gmail.com> wrote:
> Yes, as long as you have 2 different selective markers and the appropriate
> cell strain. Seehttp://www.emdbiosciences.com/html/NVG/DuetTable.html
> C.
>
> On 15 Feb 2007 12:20:34 -0800, peter <peter.ianak... from gmail.com> wrote:
>
>
>
> > Hi group,
> > I have a question regarding incompatibility of plasmids. Can one
> > bacterial cell be transformed with more than one plasmid (of the same
> > kind) and keep these plasmids. For example if one has two clones (same
> > insert, same vector) can they co-exist in the same cell (same size
> > too, only difference would be point mutation). Your thoughts are
> > invaluable and much appreciated.
> > Peter
>
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Carlo,
Exactly the same vector, wonder if one can have heterogeneous
population of point mutations in the same one bacteria.
Peter



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