(by ubwasp66 from peoplepc.com)
Fri Feb 16 18:00:35 EST 2007
You should use barrier-like tips (filter tips) (check the Gilson website for specs).
Also, you should have a dedicated set of pipettes and tip racks (preferably located in separate room from your "nucleic acid" room or inside a dedicated "clean" PCR workstation) to set up PCR reactions.
Not sure if this answers your question but hope that helps,
>From: Gaelle ayyildiz <gaelleayyildiz from yahoo.com>
>Sent: Feb 16, 2007 3:42 AM
>To: methods from magpie.bio.indiana.edu
>Subject: Pipetting, contamination....
> would u please tell me how i can pipette my regents without contaminating them with the micropipette (i.e. Gilson pipette)?
> i would really appreciate if u can show me pictures or figures of the safe way of pipetting.
> Thanx a million..
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