Pipetting, contamination....

[ubwasp66]

Gaelle,

You should use barrier-like tips (filter tips) (check the Gilson website for specs).


Also, you should have a dedicated set of pipettes and tip racks (preferably located in separate room from your "nucleic acid" room or inside a dedicated "clean" PCR workstation) to set up PCR reactions.

Not sure if this answers your question but hope that helps,


Teresa

-----Original Message-----
>From: Gaelle ayyildiz <gaelleayyildiz from yahoo.com>
>Sent: Feb 16, 2007 3:42 AM
>To: methods from magpie.bio.indiana.edu
>Subject: Pipetting, contamination....
>
>Hi all,
>   
>  would u please tell me how i can pipette my regents without contaminating them with the micropipette (i.e. Gilson pipette)?
>   
>  i would really appreciate if u can show me pictures or figures of the safe way of pipetting.
>   
>  Thanx a million..
>
>
>Gaelle
> 
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