Does autoclaving destroy DNA

newsnet customer via methods%40net.bio.net (by customer from newsnet.com)
Wed Feb 21 06:37:36 EST 2007


Hi,

Does autoclaving (121C for 20mins) DNA destroy it? What I mean by "destroy" is to break the covalent bonds between adjacent nucleotides and NOT the breaking of ionic bonds between the two strands. I argue that 121C is well above the melting temperature of DNA (95C), which should do the trick.

Keep your answers short and to the point please.

Cheers,
ST 


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