Does autoclaving destroy DNA
jg374 from cam.ac.uk
(by jg374 from hermes.cam.ac.uk)
Wed Feb 21 09:54:32 EST 2007
> Does autoclaving (121C for 20mins) DNA destroy it? What I mean by "destroy" is to break the covalent bonds between adjacent nucleotides and NOT the breaking of ionic bonds between the two strands. I argue that 121C is well above the melting temperature of DNA (95C), which should do the trick.
> Keep your answers short and to the point please.
No. Although it break a lot of it into fragments <100mers. The melting
temp refers ot the inter-strand bonds so is not relevant.
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