Purifying PCR product from 2% agarose gel

StewJW via methods%40net.bio.net (by stewjw from gmail.com)
Wed Feb 21 11:58:33 EST 2007


Invitrogen also have a gel extraction kit catalogue K2100-12, see
manual which has a trouble shooting guide which maybe helpful.

http://www.invitrogen.com/content/sfs/manuals/purelink_gelextraction_man.pdf

They don't say what their gel solubilisation agent is.

Included is an interesting recovery table:

100 bp 1 μg 42.9 ± 10.7 %
400 bp 0.5 μg 95.4 ± 8.8 %
965 bp 0.5 μg 36.3 ± 5.8 %
5.4 Kb 1 μg 63.2 ± 12.6 %




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