Purifying PCR product from 2% agarose gel

[Jose de las Heras]

"Yoram Gerchman" <gerchman from research.haifa.ac.il> wrote in message 
news:mailman.32.1171995025.5139.methods from net.bio.net...
>
> I have noticed Qiagene kits (as well as others) behave badly when the wash
> buffer lose its EtOH (like by evaporation. Its enough to have someone 
> leave the
> cap not tight to have this happen. Try to add some 100% EtOH to the wash 
> buffer.
> Yoram

?

The wash buffer is 80% EtOH... I would imagine that an awful lot of 
evaporation will have to occur before it becomes a problem.

Jose