Pipetting, contamination....

peter via methods%40net.bio.net (by peter.ianakiev from gmail.com)
Thu Feb 22 16:54:16 EST 2007


> Jeez, I've done this experiment yesterday:
> 1. Took a 1 ml Pipetman, set it to 1 ml and pipetted overnight E.coli
> culture up and down 20 times.
> 2. Immediately after that, changed a tip, took a bottle of sterile
> Terrific Broth, pipetted 20 times, set at 37C for 20 hours.
>
> The result: no signs of bacterial growth today ==> no aerosol
> contamination.
>
> DK

Seems like a good experiment, I just wonder what volume of the TB
media you used?
Peter



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