IEC cell transfection

[nanooe from gmail.com]

On Feb 22, 12:46 am, d... from no.email.thankstospam.net (DK) wrote:
> In article <mailman.54.1172102100.5139.meth... from net.bio.net>, anita balakrishnan <camelot... from yahoo.com> wrote:
>
> >Hi,
>
> >I'm trying to transfect (both transient and stable)
> >the IEC 6 cell line. I've tried Effectene and
> >Lipofectamine but both don't seem to work. Does anyone
> >have any good protocols for transfecting these cells?
> >The protocol that I've been using works for all other
> >cell lines but not for this one.
>
> Tryelectroporation.Electroporationworks for all cell types.
> Google +"IEC 6" +eletroporation.
>
> DK

If you have access to a microporator then this should work well with
IEC6 cells. All transfections we have put through it since we got it
have worked well. We got ours from Labtech International in the UK but
I don't think they build it