Protein precipitation from DNA samples
(by pow.joshi At gmail.com)
Mon Jan 8 15:28:47 EST 2007
On 1/7/07, Dr. Amir H. Shemirani <amir At dote.hu> wrote:
> I prepared DNA samples from paraffin embeded tissues by a Roche kit.
> It has a high protein concentration and I cannot use my samples for
> PCR.Please help.
would re-precipitation help, I wonder.
If your tissue is fixed in something like paraformaldehyde or
formaldehyde, there is extensive crossliking of proteins and DNA/RNA
as well, which would make all procedures difficult.....
> Dr. Amir-Houshang Shemirani
> Clinical Biochemistry & Molecular Pathology Department
> Debrecen University
> Tel: +36 52 340 006
> Fax: +36 52 417 631
> URL: www.kbmpi.hu
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