Protein precipitation from DNA samples

Christian Praetorius via methods%40net.bio.net (by prae At gmx.net)
Tue Jan 9 05:27:42 EST 2007


"Pow Joshi" <pow.joshi At gmail.com> wrote:

>If your tissue is fixed in something like paraformaldehyde or
>formaldehyde, there is extensive crossliking of proteins and DNA/RNA
>as well, which would make all procedures difficult.....

Reversing of crosslinks made by formaldehyde is possible and routinely
done when you isolate samples for chromatin immunoprecipitation.

Christian

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