(no subject)

[sheeja At spices.res.in]

dear all
When i am trying to amplify the 16 s rRNA gene (1.5 kb), I am sometimes
getting it while i am sometimes not. the bands are very faint on loading 5
microlitre of pcr product. i used to get nice thick bands earier, but not
now, i use 12o pm primer each, and all other std conditions for PCR, the
denaturation temp is 92 for 2 min and annealing is 55 for 1 min. is it
okay
please some one suggest me a good way out
thanks in advance
tes