Subject: Blocking biotin labeled blots with BSA?

Steve Crampton via methods%40net.bio.net (by scrampto At uci.edu)
Thu Jan 11 13:34:12 EST 2007


Hi Wolfgang,
If you are worried about background and are not sure if there is biotin in
your BSA, Why don't you just take a blank membrane, block it with BSA, and
incubate with the peroxidase to see if you have background or not.  You
would not have to waste your samples on a test.
Best regards
Steve Crampton
Molecular Biology & Biochemistry
University of California, Irvine
3113 McGaugh Hall
Irvine, CA 92697-3900
lab (949) 824-8143
fax (949) 824-8551
scrampto At uci.edu



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