(by ivanoov At gmail.com)
Fri Jan 12 04:53:09 EST 2007
There are a lot of published protocols. Basically you should prepare
5-10 % Chelex suspension and then mix with your sample + boiling
10-20min. Below is a link to such protocol.
The result depends on the type of sample, e.g tissue, body fluid etc.
If you want to detect small copy DNA this method is not appropriate.
Hope this helps a little
Giorgos Goras wrote:
> Hi. I want to use Chelex-100 (Sigma Corp.) for extraction of DNA after using liquid nitrogen. I don't know exactly what to do and how to use it. I think it needs continuous mixing and sterilization is very importrant.
> If someone could help me by giving me more details about this would help me very much. Thank you!
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