ccdB insensitive dam/dcm minus E.coli strain

Josmar via (by josmarlangner At
Mon Jan 15 04:38:32 EST 2007

Dear All,
to prepare dam/dcm unmethylated DNA of Gateway vector constructs I am
in need of a dam/dcm minus E.coli strain that is insensitive to ccdB
either by carrying ccdA or gyrA462. Does anyone know of such a strain
or made it in the lab?

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