RAPD breakdown

Manuel Fernando Ariza Botero via methods%40net.bio.net (by mfarizab At unal.edu.co)
Mon Jan 15 16:55:56 EST 2007


Check your PCR DNTPs and prepare a new batch. Hopefully it helps!!
bye
Fernando Ariza 

----- Mensaje original -----
De: Anajli Thomas <anjalirthomas At gmail.com>
Fecha: Lunes, Enero 15, 2007 8:11 am
Asunto: RAPD breakdown

> Sir,
>     The RAPD that we had been doing for the past 1 year in our 
> lab has
> suddenly stopped. There were absolutely no bands in the beginning. We
> increased the concentration of the MgCl2 and now there are some 
> very faint
> bands. Unfortunately it is not good enough for scoring. Could you 
> pleasehelp out.
> 
> Sincerely
> 
> Anjali
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