Fixation for PIP2 immunofluorescence

Tom Anderson via methods%40net.bio.net (by ucgatan At ucl.ac.uk)
Tue Jan 16 09:47:51 EST 2007


Hi,

Has anyone done any immunofluorescence against PIP2? If so, how did you
fix the cells?

I have a protocol involving a three-hour formaldehyde/glutaraldehyde
double fixation on ice, but if it was possible to do it at room
temperature or 37 C, at least to begin with, that would be a lot better
for the cells.

Thanks,
tom

-- 
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264   (f) +44 (20) 76797805   (e) thomas.anderson At ucl.ac.uk



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