cDNA stability?

anton via methods%40net.bio.net (by noplok At jskhkjgvdskj.kj)
Wed Jan 17 11:16:14 EST 2007


"Jose McNach" <josenet At tiscali.co.uk> skrev i en meddelelse 
news:1168972999.949719.301750 At v45g2000cwv.googlegroups.com...
>
> I normally synthesise my cDNA from RNA using Invitrogen's SuperscriptII
> reverse transcriptase, and when done I store it at -20oC. I just make
> dilutions from that stock for semiquantitative PCR and it generally
> works well, and I can go back to the stocks months later and it's still
> okay.
>
> Last November (2 months ago) I prepared a batch of cDNAs, calibrated
> them (the dilutions), did a bunch of PCRs, and this time I stored it at
> -80, together with the RNA. No particular reason.
> A month later I went back to use some of it, and failed. Then Xmas came
> and I didn't try again till today... yep, it definitely does not work
> anymore.
>
> I think the fact I stored them at -80 and failed is a coincidence...
> but I *never* have had this sort of problem before so I'm trying to be
> open minded...
>
> Has anybody experienced any problems with cDNA storage at -80?
>
> I wonder if freeze-thawing is more harmful at -80 than -20... yet I
> haven't experienced any problems at all with several rounds of
> freeze-thawing at -20 in the past...
>

Hi.
We store ALL our cDNA samples at -80, on our hands it is more stable here 
compared to minus 20! 




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