cells in culture

Zhonglin Chai via methods%40net.bio.net (by Zhonglin.Chai At baker.edu.au)
Wed Jan 24 22:45:58 EST 2007


HEK cells seem to be very sensitive to the temperature. Try not to use cold PBS or medium, always prewarm them (37oC) before use for HEK. Another possibility is the coating of vessels. Of course, they will not attach in medium without FBS...

Hope this of help.

Z Chai



-----Original Message-----
From: methods-bounces At oat.bio.indiana.edu
[mailto:methods-bounces At oat.bio.indiana.edu]On Behalf Of Pow Joshi
Sent: Thursday, 25 January 2007 10:04 AM
To: methods At magpie.bio.indiana.edu
Subject: cells in culture


Hi Folks,

this is sheer desperation: I was wondering if anyone would have any
idea why some cells (in this case HEK) are having problems attaching
to the culture plate, and then die a sad horrible death.... all
possible explanations are welcome including: "Pow, you've lost your
touch at Tissue culture, even if you've been doing it for half your
life". Thanks a bunch

pow

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