cells in culture

jg374 At cam.ac.uk via methods%40net.bio.net (by jg374 At hermes.cam.ac.uk)
Thu Jan 25 04:50:11 EST 2007

Did you trypsinse them too much? I generally use citric saline for 293s as 
I find it leaves them happier.

On Wed, 24 Jan 2007, Pow Joshi wrote:
> this is sheer desperation: I was wondering if anyone would have any
> idea why some cells (in this case HEK) are having problems attaching
> to the culture plate, and then die a sad horrible death.... all
> possible explanations are welcome including: "Pow, you've lost your
> touch at Tissue culture, even if you've been doing it for half your
> life". Thanks a bunch

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