cells in culture

[jg374 At cam.ac.uk]

Did you trypsinse them too much? I generally use citric saline for 293s as 
I find it leaves them happier.

On Wed, 24 Jan 2007, Pow Joshi wrote:
>
> this is sheer desperation: I was wondering if anyone would have any
> idea why some cells (in this case HEK) are having problems attaching
> to the culture plate, and then die a sad horrible death.... all
> possible explanations are welcome including: "Pow, you've lost your
> touch at Tissue culture, even if you've been doing it for half your
> life". Thanks a bunch