cells in culture

[Pow Joshi]

Thanks, Wolfgang....that histoire was comforting, knowing that I
was'nt the only one.... I hope your cells grow hale, hearty and well
.... in the meanwhile I am trying to grow another batch, and am hoping
these are happy, and don't catch the fever of the previous ones.....
pow
On 25 Jan 2007 15:12:58 -0800, WS <novalidaddress At nurfuerspam.de> wrote:
> Dear Pow,
>
> you have may compassion. I took mine out from liquid nitrogen after
> freezing them over the holidays and they simply refused to grow, they
> look terrible indeed. Same for BOSC23 (a 293 derivative). 3T3 mouse
> fibroblasts and H4 rat hepatoma in contrast grow more than well.I
> treated all of them the same way before christmas (trypsinize, spn
> down, resuspend, freeze), used the same freezing medium (90% FCS, 10%
> DMSO) and resuspended all of them in DMEM high glucose, 5% FCS wis P/S
> as usual. 293 HEK should grow in almost everything, actually.
>
> Must be either the moon phase, not giving them christmas presents or
> reading them good night fairy tales or simply a common cold (as I just
> had one and 293 are humans, too). Couldn't hear them cry or sneeze yet,
> however.
>
> Tried to wash away the dead ones, trypsinized them and gave them fresh
> food. Will have a look tomorrow and maybe take a new batch out of the
> cold if necessary.
>
> Maybe you have some conditioned medium handy (S/N of almost any healthy
> confluent cells, passed through a .2 or .45microns filter), that
> sometimes works, too.
>
> Good luck!
>
> Wolfgang
>
> On Jan 25, 12:04 am, "Pow Joshi" <pow.jo... At gmail.com> wrote:
> > Hi Folks,
> >
> > this is sheer desperation: I was wondering if anyone would have any
> > idea why some cells (in this case HEK) are having problems attaching
> > to the culture plate, and then die a sad horrible death.... all
> > possible explanations are welcome including: "Pow, you've lost your
> > touch at Tissue culture, even if you've been doing it for half your
> > life". Thanks a bunch
> >
> > pow
>
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