Positive control for Myc antibody

fabianh from umich.edu via methods%40net.bio.net (by fabianh from umich.edu)
Thu Jul 5 17:09:41 EST 2007


Hi,
I will have a His-Myc tagged fusion protein which I want to detect on 
Western Blots with a purchased Myc antibody. What should I use as a 
positive control? (I don't have any other protein with Myc tag 
available!)I was told that for example Hela cell lysate would be a 
possible control but how is Myc expressed in these cells and what size 
will it have?
I am thankful for any ideas!
Fabian



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