Positive control for Myc antibody
fabianh from umich.edu
(by fabianh from umich.edu)
Thu Jul 5 17:09:41 EST 2007
I will have a His-Myc tagged fusion protein which I want to detect on
Western Blots with a purchased Myc antibody. What should I use as a
positive control? (I don't have any other protein with Myc tag
available!)I was told that for example Hela cell lysate would be a
possible control but how is Myc expressed in these cells and what size
will it have?
I am thankful for any ideas!
More information about the Methods