fix and permeabilize Cells

aakash via methods%40net.bio.net (by noncodingRNAs from gmail.com)
Mon Jul 9 11:08:02 EST 2007


Hi Freinds
I would be glad if any of you can share your experience about how to
fix and permeabilize GFP expressing cells without quenching/killing
the GFP for cell cycle analysis using PI. GFP is not fusion protein. I
used paraformaldehyde and ethonol, but i loose complete gfp
expression.

Thank you



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