Concentrating protein lysates

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Tue Jul 10 14:53:00 EST 2007


Am 02.07.2007, 20:04 Uhr, schrieb DK <dk from no.email.thankstospam.net>:

>> I'm working with a new cell line that's giving me very dilute lysates  
>> (even with a vastly reduced volume of lysis buffer),

> Google for ethanol/chloroform precipitation. Just what you need.

You probably mean the Wessel & Flügge method, which uses methanol and  
chloroform. Alternatively, one might use phenol/ether (Sauvé et al.)


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