(by legatek from hotmail.com)
Tue Jul 17 00:35:17 EST 2007
Yvonne Couch wrote:
> Hi all,
> I was to run a luciferase assay to see how much ATP my cells are producing.
> For this I thought I would take an aliquot of medium from each set of
> treated cells and use it in the Invitrogen ATP-determination kit which
> employs luciferase.
What makes you think your cells are secreting ATP into the medium?
Wouldn't it make sense to make a lysate, and therefore concerns about
medium are no longer necessary?
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