Luciferase

[Kyle Legate]

Yvonne Couch wrote:
> Hi all,
> 
> I was to run a luciferase assay to see how much ATP my cells are producing.
> For this I thought I would take an aliquot of medium from each set of
> treated cells and use it in the Invitrogen ATP-determination kit which
> employs luciferase.
> 

What makes you think your cells are secreting ATP into the medium?
Wouldn't it make sense to make a lysate, and therefore concerns about 
medium are no longer necessary?