Luciferase

Kyle Legate via methods%40net.bio.net (by legatek from hotmail.com)
Tue Jul 17 00:35:17 EST 2007


Yvonne Couch wrote:
> Hi all,
> 
> I was to run a luciferase assay to see how much ATP my cells are producing.
> For this I thought I would take an aliquot of medium from each set of
> treated cells and use it in the Invitrogen ATP-determination kit which
> employs luciferase.
> 

What makes you think your cells are secreting ATP into the medium?
Wouldn't it make sense to make a lysate, and therefore concerns about 
medium are no longer necessary?


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