Merlin maxiprep help?
(by silvergrin from gmail.com)
Tue Jul 17 12:59:22 EST 2007
Hello! My sincerest apologies if you get this twice; I could really
use some help here and I didn't see my first email show up...
I found this list by looking for Merlin Maxiprep protocol
info. It was posted a *long* time ago (1994) but you can still find
the protocol here:
I've been put in charge of making the Merlin solutions. When we first
started using the assay, a couple different people had made them.
Everything worked pretty well. We ran low on Merlin I, II, and IV, so
I made up a more recently, and, horrors, the prep stopped working. D:
The problem is that DNA is not precipitating during the spin-down
after adding isopropanol to the filtered lysate.
I think the problem must lie with either buffer I or buffer II; we are
still using the old stock of buffer III, from when the assay was
I've already compared notes with the person who made up buffer I the
first time (seems to be the same), re-made buffer I in case I got
something horribly screwed up, and also tried a new solution of buffer
II. Still no crude DNA pellet at the end of the spin. Does anyone
know what the problem could be?
Thanks so much!
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