Running range of bromophenolblue and xylene cyanol?

Jose de las Heras via methods%40net.bio.net (by josenet from tiscali.co.uk)
Tue Jul 17 16:28:22 EST 2007


"Peter Frank" <peter_frankde from yahoo.de> wrote in message 
news:it9q93his3o3fg30ackccer2gvoiqohq7s from 4ax.com...
> "Jayakumar, R" <R.Jayakumar from roswellpark.org> wrote:
>
>>Depends on agarose percentage.  But generally, BPB - 300bp and XC - 3-4
>>kb.
>>Jay
>
> Thanks for the info. That was what I was looking for. Couldn't find a
> reference on the Web. Agarose percentage varies here but is usually
> around 1,5 %.
>
> Peter
>
>
>>-----Original Message-----
>>From: methods-bounces from oat.bio.indiana.edu
>>[mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of Peter Frank
>>Sent: Tuesday, July 17, 2007 1:59 PM
>>To: methods from magpie.bio.indiana.edu
>>Subject: Running range of bromophenolblue and xylene cyanol?
>>
>>Hi,
>>
>>The loading buffer for my DNA sample contains the dyes bromophenolblue
>>and xylene cyanol. Can anyone tell me in what bp (base pair) range these
>>dyes run in agarose gels for DNA analysis (i.e. DNA fragments of what
>>size would run along with the bromophenolblue front or the xylene cyanol
>>front)?
>>
>>Regards,
>>Peter

it's simpler to check, and then you'll be sure :-)

Jose 




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