Luciferase

Kyle Legate via methods%40net.bio.net (by legatek from hotmail.com)
Wed Jul 18 01:32:26 EST 2007


Han wrote:
> 
> Making a cell lysate has the "problem" of mixing all cellular 
> compartments, both those with ATP and those with nucleotidases and 
> phosphatases.  Therefore, again time is of the essence.  The luciferase 
> has to see the ATP before the other metabolizing enzymes, AND the 
> luciferase signal has to be recorded before either luciferase or other 
> enzymes have consumed all the ATP.
> 
> 
It sounds like using luciferase to measure ATP production is a little 
problematic. How about coupling it to a more stable, colourimetric 
reaction? It might be less sensitive, but more reproducible.


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