(by legatek from hotmail.com)
Wed Jul 18 01:32:26 EST 2007
> Making a cell lysate has the "problem" of mixing all cellular
> compartments, both those with ATP and those with nucleotidases and
> phosphatases. Therefore, again time is of the essence. The luciferase
> has to see the ATP before the other metabolizing enzymes, AND the
> luciferase signal has to be recorded before either luciferase or other
> enzymes have consumed all the ATP.
It sounds like using luciferase to measure ATP production is a little
problematic. How about coupling it to a more stable, colourimetric
reaction? It might be less sensitive, but more reproducible.
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