RE to Jim 3975 - Strong contamination with ethidium bromide

peter via methods%40net.bio.net (by peter.ianakiev from gmail.com)
Fri Jun 8 10:57:32 EST 2007


On Jun 8, 6:15 am, "Prof. Piero Sestili" <sest... from uniurb.it> wrote:
> Dear Jim 3975,
>
> of course, and as anybody in this field knows, you're right: my story
> was simply to tranquillize this poor undergraduate student...I think
> that your intervention got the opposite result!  Another very relevant
> aspect of this specific case (mine and undergraduate's one) is that we
> are speaking of a SINGLE exposure, far less effective in terms of
> carcinogenicity as compared to CHRONIC exposure.
> By the way, if there's anybody who stained his hands with EB more than
> 16 years ago, please come on and tell us your story!
>
> Prof. Piero Sestili
> Istituto di Farmacologia e Farmacognosia e
> Centro di Ricerca sull'Attività Motoria
> Università degli Studi di Urbino "Carlo Bo"
> Via "I Maggetti" 26
> 61029 URBINO (PU)
> Tel. 0722 303414; 0722 305524
> Fax 0722 303401
>
> -----Messaggio originale-----
> Da: methods-boun... from oat.bio.indiana.edu
> [mailto:methods-boun... from oat.bio.indiana.edu] Per conto di Jim 3975
> Inviato: giovedì 7 giugno 2007 22.00
> A: meth... from magpie.bio.indiana.edu
> Oggetto: Re: Strong contamination with ethidium bromide
>
> To get cancer, 16 years is nothing. People who start smoking at 16 don't
> get
> lung cancer until 40, 50 years later. (Average age of lung cancer death
> is
> ~70)
> Bruce Ames showed in a PNAS paper in the seventies that smoking is more
> mutagenic than ethidum bromide (using fairly realistic doses of both).
>
>
>
> >"Prof. Piero Sestili" <sest... from uniurb.it> wrote in message
> >news:mailman.1372.1181135432.5139.methods from net.bio.net...
> >Nothing to do with the past contamination!  Probably few EB molecules
> >penetrated to the germinative layer of your skin and most of it was
> >cleared by corneum.  Just forget it!  16 years ago I noticed an EB
> stain
> >on my right hand, due to unexpectedly broken gloves: I'm still here,
> >writing with the same hand....
> >For the future simply wear two pairs of lattice gloves when using EB:
> >normal gloves do not protect so much...and go on with your PhD
>
> _______________________________________________
> Methods mailing list
> Meth... from net.bio.nethttp://www.bio.net/biomail/listinfo/methods

EtBr is overrated IMHO, Friend of mine spilled about 250ml on himself,
did not change clothes for a few hours and 14 years later he is fine,
has 3 children and all is as normal as it can be. Don't stress
yourselves with EtBr/gloves etc. In my experience most contaminations
happened when people wore gloves....just wear gloves when handle
acrilamide , DMSO, PMSF etc.



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