Tween-20 versus Triton-X 100

lowenbrau via methods%40net.bio.net (by customer from newsnet.com)
Sat Jun 9 09:16:31 EST 2007


> Hi all-
> I am trying to figure out my protocol for fluorescence in situ based a 
> protocol for not fluorescent in situ.  The former uses Triton-X 100 while 
> the latter uses Tween 20.  Is there is big difference between these?  Will 
> one of them give me better results?
>
> Thanks!
> Wendy

I believe they r both surfactants, so they will destroy the cell plasma 
membrane. However, there may be subtle differences between the two.

Hope that helps.

ST




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