Tween-20 versus Triton-X 100
lowenbrau
via methods%40net.bio.net
(by customer from newsnet.com)
Sat Jun 9 09:16:31 EST 2007
> Hi all-
> I am trying to figure out my protocol for fluorescence in situ based a
> protocol for not fluorescent in situ. The former uses Triton-X 100 while
> the latter uses Tween 20. Is there is big difference between these? Will
> one of them give me better results?
>
> Thanks!
> Wendy
I believe they r both surfactants, so they will destroy the cell plasma
membrane. However, there may be subtle differences between the two.
Hope that helps.
ST
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