RE to Jim 3975 - Strong contamination with ethidium bromide
(by jim3975 from nospamTRTnet.nl)
Sat Jun 9 04:46:06 EST 2007
Well I have been working with EBr in a rather sloppy way (some of my
colleagues would use stronger terms) since like thirty years. 'Finger
cancer' (as someone else in this thread called it) is not among the
problems I currently have, nor of any of my lab colleagues. (I must say I
took care never to ingest any, I mean washing my hands before eating etc. )
My reference to the PNAS paper was as sloppy as my way of working with EBr.
The paper mentions Ebr, but not cigarette smoke. That must have been a
different paper, I can't find it right now.
Even if carcinogenity would be similar, it would be odd - just as you are
correctly pointing out - to panick about having smoked say, 50 cigarettes
_only one time_.
BTW, it is funny that there do not seem to be reports of studies of the
effect of EtBr on animals. After all, strong mutagens can be helpful in
"Prof. Piero Sestili" <sestili from uniurb.it> wrote in message
news:mailman.1419.1181314246.5139.methods from net.bio.net...
> Dear Jim 3975,
> of course, and as anybody in this field knows, you're right: my story
> was simply to tranquillize this poor undergraduate student...I think
> that your intervention got the opposite result! Another very relevant
> aspect of this specific case (mine and undergraduate's one) is that we
> are speaking of a SINGLE exposure, far less effective in terms of
> carcinogenicity as compared to CHRONIC exposure.
> By the way, if there's anybody who stained his hands with EB more than
> 16 years ago, please come on and tell us your story!
> Prof. Piero Sestili
> Istituto di Farmacologia e Farmacognosia e
> Centro di Ricerca sull'Attività Motoria
> Università degli Studi di Urbino "Carlo Bo"
> Via "I Maggetti" 26
> 61029 URBINO (PU)
> Tel. 0722 303414; 0722 305524
> Fax 0722 303401
> -----Messaggio originale-----
> Da: methods-bounces from oat.bio.indiana.edu
> [mailto:methods-bounces from oat.bio.indiana.edu] Per conto di Jim 3975
> Inviato: giovedì 7 giugno 2007 22.00
> A: methods from magpie.bio.indiana.edu
> Oggetto: Re: Strong contamination with ethidium bromide
> To get cancer, 16 years is nothing. People who start smoking at 16 don't
> lung cancer until 40, 50 years later. (Average age of lung cancer death
> Bruce Ames showed in a PNAS paper in the seventies that smoking is more
> mutagenic than ethidum bromide (using fairly realistic doses of both).
>>"Prof. Piero Sestili" <sestili from uniurb.it> wrote in message
>>news:mailman.1372.1181135432.5139.methods from net.bio.net...
>>Nothing to do with the past contamination! Probably few EB molecules
>>penetrated to the germinative layer of your skin and most of it was
>>cleared by corneum. Just forget it! 16 years ago I noticed an EB
>>on my right hand, due to unexpectedly broken gloves: I'm still here,
>>writing with the same hand....
>>For the future simply wear two pairs of lattice gloves when using EB:
>>normal gloves do not protect so much...and go on with your PhD
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