Tween-20 versus Triton-X 100

WS via (by novalidaddress from
Sat Jun 9 16:26:10 EST 2007

Hi Wendy,

as they both seem to work, I'd go ahead with any of them. Usually
people use what's available on the shelf, so there will be different
protocols for the same purpose. Both are nonionic detergents with
quite similar properties.

Normal preparations of TritonX100 (but not Tween 20 AFAIK) are known
to have some absorption in the UV range as Triton has an aromatic
ring, but that should not matter regarding fluorescence, as you
probably will use "ordinary" dyes which work in the visible range. If
you expect any problems or just in case, run a fluorescence spectrum
at working concentration.

Best regards,


More information about the Methods mailing list