cDNA Synthesis

[Jose de las Heras]

As Peter said, don't buy kits. I also use SuperscriptII from Invitrogen.
The oligo(dT) I just order from my favourite oligo synthesis company... save 
you a fortune.
Random hexamers you can buy from different sources, but I wouldn't be 
surprised if you couldn't get them cheaper also by ordering directly with 
your oligo company. Worth checking...

Jose



"Yvonne Couch" <yvonne.couch from dpag.ox.ac.uk> wrote in message 
news:mailman.1444.1181583872.5139.methods from net.bio.net...
Hi all,

I'm a PCR novice and am looking for a cDNA synthesis kit but they all seem
to do so many at so many different prices.  I want a first strand synthesis
kit with OligodT and random hexamer primers.  The one we have is around £200
for 200 rxn from Applied Biosystems.  Anyone know of anything cheaper?

Thanks

Yvonne