How to get a single stranded DNA as long as 2-3kb?
(by hzhen from freeuk.com)
Mon Jun 11 14:57:39 EST 2007
On Mon, 11 Jun 2007 19:40:59 GMT, Nick Theodorakis
<nick_theodorakis from hotmail.com> wrote:
>WANG XF wrote:
>> Hi, everyone!
>> I have a plasmid containing a 3kb fragment, and now want to prepare a
>> stranded DNA as long as 2-3kb. Could anyone give a good suggestions with a
>> simple method? Or you can provide a reference.
>> Thank you so much !
>> WANG XF
>If it's in Bluescript or a similar vector with an f1 origin you can
>recover single stranded phagemids after infection of a suitable host
>with a helper virus. See the manual for details:
Isn't pBluescript the one that produce the ghost band of ssDNA when
you do a normal DNA prep?
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