How to get a single stranded DNA as long as 2-3kb?

ChenHA via methods%40net.bio.net (by hzhen from freeuk.com)
Mon Jun 11 15:03:13 EST 2007


On Mon, 11 Jun 2007 20:57:39 +0100, ChenHA <hzhen from freeuk.com> wrote:

>On Mon, 11 Jun 2007 19:40:59 GMT, Nick Theodorakis
><nick_theodorakis from hotmail.com> wrote:
>
>>WANG XF wrote:
>>> Hi, everyone!
>>> 
>>> I have a plasmid containing a 3kb fragment, and now want to prepare a 
>>> single
>>> stranded DNA as long as 2-3kb. Could anyone give a good suggestions with a
>>> simple method? Or you can provide a reference.
>>> 
>>> Thank you so much !
>>> 
>>> WANG XF
>>
>>If it's in Bluescript or a similar vector with an f1 origin you can 
>>recover single stranded phagemids after infection of a suitable host 
>>with a helper virus. See the manual for details:
>>
>><http://www.stratagene.com/manuals/212205.pdf>
>>
>>Nick
>
>
>Isn't pBluescript the one that produce the ghost band of ssDNA when
>you do a normal DNA prep? 

Perhaps I misremember, was it XL1 blue cells that produces the ghost
band of ssDNA instead?   




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