How to get a single stranded DNA as long as 2-3kb?
(by nick_theodorakis from hotmail.com)
Mon Jun 11 15:22:57 EST 2007
> Perhaps I misremember, was it XL1 blue cells that produces the ghost
> band of ssDNA instead?
I seem to remember a discussion about NaOH irreversibly altering the
plasmid conformation to generate a ghost band during alkaline preps, but
perhaps there is more than one kind of ghost band.
nick_theodorakis from hotmail.com
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