How to get a single stranded DNA as long as 2-3kb?

Nick Theodorakis via (by nick_theodorakis from
Mon Jun 11 15:22:57 EST 2007

ChenHA wrote:
> Perhaps I misremember, was it XL1 blue cells that produces the ghost
> band of ssDNA instead?   

I seem to remember a discussion about NaOH irreversibly altering the 
plasmid conformation to generate a ghost band during alkaline preps, but 
perhaps there is more than one kind of ghost band.


Nick Theodorakis
nick_theodorakis from
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