How to get a single stranded DNA as long as 2-3kb?

Nick Theodorakis via methods%40net.bio.net (by nick_theodorakis from hotmail.com)
Mon Jun 11 15:22:57 EST 2007


ChenHA wrote:
[...]
> 
> Perhaps I misremember, was it XL1 blue cells that produces the ghost
> band of ssDNA instead?   
> 
> 

I seem to remember a discussion about NaOH irreversibly altering the 
plasmid conformation to generate a ghost band during alkaline preps, but 
perhaps there is more than one kind of ghost band.

Nick

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Nick Theodorakis
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