How to get a single stranded DNA as long as 2-3kb?

[peter]

On Jun 11, 9:34 am, "WANG XF" <wxf... from 163.com> wrote:
> Hi, everyone!
>
> I have a plasmid containing a 3kb fragment, and now want to prepare a single
> stranded DNA as long as 2-3kb. Could anyone give a good suggestions with a
> simple method? Or you can provide a reference.
>
> Thank you so much !
>
> WANG XF

Yep, Do the PCR of your fragment, purify trough a column (or dilute
1:100) and make another round of PCR with just one of the primers
(that will give your desired strand)
Peter