How to get a single stranded DNA as long as 2-3kb?

[peter]

On Jun 11, 2:30 pm, peter <peter.ianak... from gmail.com> wrote:
> On Jun 11, 9:34 am, "WANG XF" <wxf... from 163.com> wrote:
>
> > Hi, everyone!
>
> > I have a plasmid containing a 3kb fragment, and now want to prepare a single
> > stranded DNA as long as 2-3kb. Could anyone give a good suggestions with a
> > simple method? Or you can provide a reference.
>
> > Thank you so much !
>
> > WANG XF
>
> Yep, Do the PCR of your fragment, purify trough a column (or dilute
> 1:100) and make another round of PCR with just one of the primers
> (that will give your desired strand)
> Peter

Alternatively, you could use one of your primers phosphorylated and
after PCR you could degrade that strand with Lambda exo (from NEB)