cDNA Synthesis (Jose de las Heras)

[Jose de las Heras]

The Superscript III is an improved version of II, I think, with higher 
processivity.
I buy the II alone, it merely comes with buffer and DTT. I'm not sure how 
much more expensive the III is, but I'm pretty happy with II :-)
(standard cDNA synthesis as well as microarray labelling)

Jose

"Analia Alet" <analia_alet from intech.gov.ar> wrote in message 
news:mailman.1461.1181706908.5139.methods from net.bio.net...
> Hi
> I used to made cDNA synthesis with SperScript III from Invitrogen (cat. 
> no.
> 18080-051). I don´t know if it's the same for SuperScript II, but in the 
> III
> you have two eppendorfs, one for oligodT primers and another for random
> hexamers. It worked well for me.
>
> Best regards
> Analía
>
> As Peter said, don't buy kits. I also use SuperscriptII from Invitrogen.
> The oligo(dT) I just order from my favourite oligo synthesis company... 
> save
> you a fortune.
> Random hexamers you can buy from different sources, but I wouldn't be
> surprised if you couldn't get them cheaper also by ordering directly with
> your oligo company. Worth checking...
>
> Jose
>
>
>
> "Yvonne Couch" <yvonne.couch from dpag.ox.ac.uk> wrote in message
> news:mailman.1444.1181583872.5139.methods from net.bio.net...
> Hi all,
>
> I'm a PCR novice and am looking for a cDNA synthesis kit but they all seem
> to do so many at so many different prices.  I want a first strand 
> synthesis
> kit with OligodT and random hexamer primers.  The one we have is around 
> £200
> for 200 rxn from Applied Biosystems.  Anyone know of anything cheaper?
>
> Thanks
>
> Yvonne
>