Ghost band (was Re: How to get a single stranded DNA as long as 2-3kb?)

[peter]

On Jun 13, 8:09 am, Tom Anderson <ucga... from ucl.ac.uk> wrote:
> On Wed, 13 Jun 2007, peter wrote:
> > On Jun 12, 5:31 pm, "Jose de las Heras" <jose... from tiscali.co.uk> wrote:
> > > "peter" <peter.ianak... from gmail.com> wrote in message
>
> > >news:1181593924.822581.168770 from q75g2000hsh.googlegroups.com...
>
> > > > On Jun 11, 4:22 pm, Nick Theodorakis <nick_theodora... from hotmail.com>
> > > > wrote:
> > > >> ChenHA wrote:
>
> > > >> > Perhaps I misremember, was it XL1 blue cells that produces the
> > > >> > ghost band of ssDNA instead?
>
> > > >> I seem to remember a discussion about NaOH irreversibly altering
> > > >> the plasmid conformation to generate a ghost band during alkaline
> > > >> preps,
>
> > > > There is no such thing like "ghost" bands.
>
> > > actually, "ghost" bands do exist.
>
> > > They do seem to be related to a change in conformation of the plasmid
> > > DNA under alkaline conditions. I remember vaguely an old paper, from
> > > the 70s, describing what conformation circular DNA can take, and it
> > > was defined as "type V" DNA, or something like that...
>
> > Jose, Why do you think I should believe to a paper from the 70s?  Do you
> > know how many crappy papers were published in the past that are pure BS
> > when we look at retrospect....
>
> Significantly fewer than the number of crappy papers published recently
> that are pure BS when you look at them.
>
> But Peter, if you choose to ignore all papers from the 70s and earlier on
> principle, that's fine by me, because you're one less potential competitor
> i have to worry about.
>
> tom
>
> --
> Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
> (t) +44 (20) 76797264   (f) +44 (20) 76797805   (e) thomas.ander... from ucl.ac.uk

Tom, I can only agree with you on your observation regarding the
recent crap. I think it is also a function of the number of journals
that have to publish  certain number of pages every month, and the
pressure on the scientist to come up with funding. I don't ignore
anything that is of value Tom. I can reassure you that it would be
difficult for me to compete with you and you don't have to worry  :-)