PCR after DNA staining

[Jose de las Heras]

"Martin Downes" <Martin.Downes from nuim.ie> wrote in message 
news:mailman.1468.1181758822.5139.methods from net.bio.net...
> Is there a DNA stain that might be used (like DAPI) on plant tissue, but 
> that allows PCR to be used on the stained DNA afterwards ?

I think that methylene blue _might_ work.

I used it to stain wheat chromosomes on a slide, and microdissect specific 
chromosomes out, which later were amplified by DOP-PCR. It seemed to work, 
although I then stopped staining and just used phase contrast...

Jose